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vienna bioactivity crispr score portal50  (Addgene inc)


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    Structured Review

    Addgene inc vienna bioactivity crispr score portal50
    Vienna Bioactivity Crispr Score Portal50, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vienna+bioactivity+crispr+score+portal50/lentiCRISPR+v2+(Plasmid+%2352961)/pm38036533-271-9-20
    Average 96 stars, based on 6143 article reviews
    vienna bioactivity crispr score portal50 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    CRISPR:

    Article Title: Discovery of a Drug-like, Natural Product-Inspired DCAF11 Ligand Chemotype.
    Article Snippet: The BRD4-BFP reporter was engineered by inserting BRD4 short isoform (amino acids 1-719; Twist Bioscience) and mTagBFP into a modified pRRL lentiviral backbone48. mTagBFP2pBADwas a gift fromMichael Davidson (Addgene plasmid # 54572)49. sgRNA-resistant DCAF11 wild type and triple alanine mutant (C443A, C460A, C485A) cDNAs used for knockout/rescue studies were custom synthesized by Twist Biosciences, coupled to a 3xFLAG-Tag and cloned into a pRRL lentiviral vector expressing iRFP670 for flow cytometry detection. .. DCAF11- and FIP200 (RB1CC1)-targeting sgRNAs were designed using the Vienna Bioactivity CRISPR score portal50 (www.vbc-score.org) and were cloned into pLentiCRISPRv2 (Addgene #52961) or pLenti-U6-sgRNA-IT-EF1αs-Thy1.1-P2A-NeoR (gift from Johannes Zuber) following standard protocol25,51. ..

    Clone Assay:

    Article Title: Discovery of a Drug-like, Natural Product-Inspired DCAF11 Ligand Chemotype.
    Article Snippet: The BRD4-BFP reporter was engineered by inserting BRD4 short isoform (amino acids 1-719; Twist Bioscience) and mTagBFP into a modified pRRL lentiviral backbone48. mTagBFP2pBADwas a gift fromMichael Davidson (Addgene plasmid # 54572)49. sgRNA-resistant DCAF11 wild type and triple alanine mutant (C443A, C460A, C485A) cDNAs used for knockout/rescue studies were custom synthesized by Twist Biosciences, coupled to a 3xFLAG-Tag and cloned into a pRRL lentiviral vector expressing iRFP670 for flow cytometry detection. .. DCAF11- and FIP200 (RB1CC1)-targeting sgRNAs were designed using the Vienna Bioactivity CRISPR score portal50 (www.vbc-score.org) and were cloned into pLentiCRISPRv2 (Addgene #52961) or pLenti-U6-sgRNA-IT-EF1αs-Thy1.1-P2A-NeoR (gift from Johannes Zuber) following standard protocol25,51. ..



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    96
    Addgene inc vienna bioactivity crispr score portal50
    Vienna Bioactivity Crispr Score Portal50, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vienna+bioactivity+crispr+score+portal50/lentiCRISPR+v2+(Plasmid+%2352961)/pm38036533-271-9-20
    Average 96 stars, based on 1 article reviews
    vienna bioactivity crispr score portal50 - by Bioz Stars, 2026-09
    96/100 stars
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